AflII R0520S from New England Biolabs

Product Specs
ItemAflII
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberR0520S
Concentration10 u/µL
Quantity2000 units
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeRestriction Enzyme
Temperature-20 C

Sequence

C/TTAAG

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesElifeDecember 14, 2023Nicole A. Ellis, Kevin S. Myers, Jessica Tung, Anne Davidson Davidson Ward, Kathryn Johnston, Katherine E. Bonnington, Timothy J. Donohue, Matthias P. Machner(Macherey-Nagel 740499.250) for plasmid isolation and elution in water. A NotI-HF (NEB R3189S) and AflII (NEB R0520S) double digest was performed on the four now recombined eluates, as well as one aliquot of the circular

(2) Cis -regulatory control of transcriptional timing and noise in response to estrogenbioRxivMarch 18, 2023Matthew Ginley-Hidinger, Hosiana Abewe, Kyle Osborne, Katelyn L. Mortenson, Alexandra Richey, Erin M. Wissink, John Lis, Xiaoyang Zhang, Jason Gertzreagent:DNA ratio. dCas9-VP16(10x) plasmid was linearized with restriction enzyme AflII (New England Biolabs R0520S). Successful integration of the dCas9-VP16(10x) plasmid was selected for using G418 (Thermo Scientific)

(3) Targeting PEA3 transcription factors to mitigate small cell lung cancer progressionOncogeneFebruary 1, 2023Shia DW, Choi W, Vijayaraj P, Vuong V, Sandlin JM, Lu MM, Aziz A, Marin C, Aros CJ, Sen C, et al.expression vector. Vector was linearized via incubation with restriction enzyme AflII (New England BioLabs, R0520S) according to manufacturer’s instructions. Spacer sequence was inserted into expression plasmid via

(4) THOR is a targetable epigenetic biomarker with clinical implications in breast cancerClinical EpigeneticsDecember 18, 2022Joana Dias Apolónio, João S. Dias, Mónica Teotónio Fernandes, Martin Komosa, Tatiana Lipman, Cindy H. Zhang, Ricardo Leão, Donghyun Lee, Nuno Miguel Nunes, Ana-Teresa Maia, et al.the gRNA target sequence into the pPlatTET-gRNA2 (TET_MO), the plasmid was digested with AflII (NEB, R0520S) through incubation at 37 °C for 3 h, followed by heat inactivation at 65 °C for 20 min. The cloning

(5) Helicobacter pylori shows tropism to gastric differentiated pit cells dependent on urea chemotaxisNature communicationsOctober 5, 2022Aguilar C, Pauzuolis M, Pompaiah M, Vafadarnejad E, Arampatzi P, Fischer M, Narres D, Neyazi M, Kayisoglu Ö, Sell T, et al.Benchling (www.benchling.com). For MUC5AC, overhangs were added to the gRNA sequence and cloned into AflII (R0520S, New England Biolabs) linearized gRNA_Cloning plasmid (41824, Addgene). A plasmid expressing hCas9 was

(6) Species-specific sensitivity to TGFβ signaling and changes to the Mmp13 promoter underlie avian jaw development and evolutioneLifeJune 6, 2022Smith SS, Chu D, Qu T, Aggleton JA, Schneider RA.Sanger sequencing and cloned into our pPIDNB custom-made plasmid (Chu et al., 2020), which was digested with AflII (R0520S, NEB, Ipswich, MA) and PstI (R3140S, NEB, Ipswich, MA), using NEBuilder HiFi DNA Assembly Master Mix.

(7) Mapping enzyme catalysis with metabolic biosensingNature CommunicationsDecember 1, 2021Linfeng Xu et al.ligation (Supplementary Fig. 1). The pTEF-2PS plasmid is linearized by DraIII and AflII (R3510S and R0520S, New England Biolabs) digestion. The library amplicons are cloned into linearized pTEF-2PS by NEBuilder

(8) An early cell shape transition drives evolutionary expansion of the human forebrainCellApril 15, 2021Benito-Kwiecinski S, Giandomenico SL, Sutcliffe M, Riis ES, Freire-Pritchett P, Kelava I, Wunderlich S, Martin U, Wray GA, McDole K, et al.CAG-fl-STOP-fl cassette, excised from the construct pT2-CAG-fl-STOP-fl-fGFP with restriction enzymes AflI (NEB, R0520S) and AscI (NEB, R0558S) and blunted with the Quick Blunting Kit (NEB, E1201). The resulting intermediate

(9) Single-strand DNA breaks cause replisome disassemblyMolecular CellMarch 1, 2021Kyle B. Vrtis, James M. Dewar, Gheorghe Chistol, R. Alex Wu, Thomas G. W. Graham, Johannes C. Walter11873580001 Nb.BbvCI,NEB,Cat# R0631S Nt.BbvCI,NEB,Cat# R0632S Nt.BspQI,NEB,Cat# R0644S AflII,NEB,Cat# R0520S NcoI,NEB,Cat# R0193L MfeI-HF,NEB,Cat# R3589S "Klenow Fragment, exo-",NEB,Cat# M0212S AlexaFluor647-maleimide,Thermo Fisher

(10) Generation of PAX7 Reporter Cells to Investigate Skeletal Myogenesis from Human Pluripotent Stem CellsSTAR protocolsDecember 18, 2020Xi H, Young CS, Pyle AD."AccuPrime Taq DNA Polymerase, high fidelity",Thermo Fisher Scientific,Cat#: 12346086 AflII,New England BioLabs,Cat#: R0520S NEBuilder HiFi DNA Assembly Master Mix,New England BioLabs,Cat#: E2621S TransIT-293 Transfection Reagent,Mirus,Cat#:

(11) Impaired flickering of the permeability transition pore causes SPG7 spastic paraplegiaEBioMedicineNovember 1, 2020Sambri I, Massa F, Gullo F, Meneghini S, Cassina L, Carraro M, Dina G, Quattrini A, Patanella L, Carissimo A, et al.pcDNA3.1 MCS-BirA(R118G)-HA vector and the PCR product of SPG7 gene were digested by the AflII (Cat# R0520S - New England Biolabs, Ipswich, MS, USA) and the EcoRI (Cat# R3101S - New England Biolabs, Ipswich,

(12) Stable integration of an optimized inducible promoter system enables spatiotemporal control of gene expression throughout avian developmentBiology openOctober 6, 2020Chu D, Nguyen A, Smith SS, Vavrušová Z, Schneider RA.Runx2, Mmp13, Cxcl14, and Gas1 were cloned into pEPIC1.1 digested with AflII (NEB, Ipswich, MA, USA, R0520S) and EcoRI (NEB, Ipswich, MA, USA, R3101S) or pPIDNB digested with AflII (NEB, Ipswich, MA, USA, R0520S)

(13) The Initiation of Meiotic Sex Chromosome Inactivation Sequesters DNA Damage Signaling from Autosomes in Mouse SpermatogenesisCurrent biology : CBFebruary 3, 2020Abe H, Alavattam KG, Hu YC, Pang Q, Andreassen PR, Hegde RS, Namekawa SH.oviduct ampulla of pseudopregnant CD-1 female mice. Pups were born and genotyped by PCR with AflII (NEB, R0520S) enzyme digestion. Animals were housed in a controlled environment with a 12-hour (h) light/12-h dark

(14) Delayed Newcastle disease virus replication using RNA interference to target the nucleoproteinBiologicalsJuly 1, 2015Jessica M. Hutcheson, Leonardo Susta, Steven L. Stice, Claudio L. Afonso, Franklin D. Westassess correct insertion of the pre-miRNA cassette (New England BioLabs (Ipswich, MA) CAT# R0156; CAT# R0520S). DNA sequencing was then used to confirm the restriction digestion results. Confirmed lentiviral vectors

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4/25/2018Diagnostic Restriction Mapping Of The Plasmid Construct With AflII Enzyme Triveni MahendrappaI have constructed a plasmid called pUC19_atpA of size 5113 bp. These constructed were transformed into E. coli DH5 alpha cells. Plasmid was prepped from E. coli DH5 alpha cells to quickly confirm ... Read Review

AflII

AflII from New England Biolabs

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AflII from New England Biolabs

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